Resistance Induction in Barley Coleoptile Cells by Intracellular pH Decline
نویسندگان
چکیده
منابع مشابه
Regulation of intracellular pH in eukaryotic cells.
The fact that cytoplasmic pH is strictly regulated has only been appreciated during the last 10 years. Eukaryotic cells clamp cytoplasmic pH at 7.0-7.4 by ion transport mechanisms and a high buffering capacity of the cytosol. The values of internal pH observed (pH 7.0-7.4) are higher than expected if the protons had been passively distributed across the cell membrane according to electrochemica...
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Cell polarization along the axis of movement is required for migration. The localization of proteins and regulators of the migratory machinery to either the cell front or its rear results in a spatial asymmetry enabling cells to simultaneously coordinate cell protrusion and retraction. Protons might function as such unevenly distributed regulators as they modulate the interaction of focal adhes...
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Cytoplasmic pH in single living specimens of Chaos carolinensis is determined microfluorometrically by measuring the ratio of fluorescence intensity of microinjected fluorescein-thiocarbamyl (FTC)-ovalbumin at two different excitation wavelengths. The probe is evenly distributed throughout, and confined to, the cytoplasm, and the fluorescence intensity ratio depends only upon pH. It is independ...
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To investigate intracellular pH (pHi) in human resistance arteries in essential hypertension, vessels were obtained from small biopsies of skin and subcutaneous fat from 14 untreated patients, and the results were compared with those from 14 matched normotensive control volunteers. Segments of isolated resistance arteries were mounted in a myograph and loaded with the pH-sensitive fluorescent d...
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Cells within solid tumors are known to exist in a microenvironment that may be acidic and depend on membrane-based mechanisms (Na+/H+ antiport and Na+-dependent Cl-/HCO3- exchanger) that regulate intracellular pH (pHi). We have used the fluorescent pH indicator 2',7'-bis-(2-carboxyethyl) 5 (and 6)-carboxyfluorescein and flow cytometry to study the distribution of pHi and the activity of these p...
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ژورنال
عنوان ژورنال: Plant and Cell Physiology
سال: 2000
ISSN: 1471-9053,0032-0781
DOI: 10.1093/pcp/pcd065